Ultrasonic treatment of Cryptosporidium oocysts.

Disinfection of Cryptosporidium parvum Oocysts in Water Using Ultrasonic Treatment

Ultrasound in a liquid phase cause mass and heat transfer across the liquid through cavitational processes which act as nanoreactors to generate unstable mechanical equilibrium. The effect of 1 MHz ultrasound on the inactivation of Cryptosporidium parvum was investigated. Continuous irradiation of ultrasound (20 min) increased temperature due to cavitational phenomena. Ultrasound irradiation of...

Comparison of 12 Techniques for Detection of Cryptosporidium oocysts

Cryptosporidium propidium monoazide-PCR, a molecular biology-based technique for genotyping of viable Cryptosporidium oocysts.

Cryptosporidium is an important waterborne protozoan parasite that can cause severe diarrhea and death in the immunocompromised. The current methods used to monitor for Cryptosporidium oocysts in water are the microscopy-based USEPA methods 1622 and 1623. These methods assess total levels of oocysts in source waters, but do not determine oocyst viability or genotype. Recently, propidium monoazi...

Cryptosporidium Oocysts USING Conventional TREATMENT filtration

ater suppliers need information about the removal efficiency of Cryptosporidium and potential surrogate parameters over a broad range of water quality and treatment conditions. These data can be obtained by tracking environmental concentrations of Cryptosporidium through full-scale treatment plants or feeding larger numbers of oocysts through pilot-scale facilities. Table 1 shows published Cryp...

Detection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay

Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples wer...